Table 3.

Ranking of datasets based on data collection and processing time and resolution.

Ranking	Dataset	Total collection + processing time (h)	Resolution (Å)	# particles (K)	Ice thickness range (nm)
1	#3b	11.9	2.8	87	10–20
2	#1b	15.5	2.8	62	10–20
3	#1	20.9	2.5	219	10–20
4	#1a	21.0	2.5	124	10–20
5	#3a	47.6	2.4	186	10–20
6	#3	48.9	2.4	205	10–20
7	#2	59.1	3.0	204	100–250
8	#2a	53.2	3.5	22	100–250
9	#2b	25.4	4.6	75	100–250

Datasets were ranked primarily on total data collection + processing time and secondarily on nominal resolution. Six out of nine datasets went to <3 Å. We find that all <3 Å reconstructions come from datasets with 10–20 nm ice thickness and that more than half of those <3 Å datasets were acquired in under 24 h. Datasets that do not go <3 Å had ice thickness measurements ranging from 100–250 nm.