Table 3. Intra-day and inter-day precision of measurements of standard solutions of dNTPs and NTPs.
| Amount (pmol, 5 μl injected) | Intra-day precision | Inter-day precision | ||
|---|---|---|---|---|
| Spiked | Found ± SD | %CV | Found ± SD | %CV |
| dCTP | ||||
| 1 | 0.86 ± 0.06 | 7.3 | 0.86 ± 0.01 | 1.6 |
| 2 | 1.86 ± 0.09 | 5.1 | 1.83 ± 0.03 | 1.8 |
| 8 | 7.16 ± 0.26 | 3.7 | 6.88 ± 0.40 | 5.8 |
| dTTP | ||||
| 1 | 1.20 ± 0.04 | 3.0 | 1.13 ± 0.05 | 4.2 |
| 2 | 1.95 ± 0.03 | 1.8 | 1.93 ± 0.01 | 0.4 |
| 8 | 8.73 ± 0.39 | 4.5 | 8.15 ± 0.51 | 6.3 |
| dATP | ||||
| 0.4 | 0.43 ± 0.01 | 2.0 | 0.33 ± 0.01 | 3.2 |
| 0.8 | 0.82 ± 0.06 | 7.4 | 0.82 ± 0.05 | 5.5 |
| 2.5 | 2.75 ± 0.10 | 3.7 | 2.55 ± 0.12 | 4.5 |
| dGTP | ||||
| 0.4 | 0.34 ± 0.04 | 12.7 | 0.35 ± 0.04 | 10.5 |
| 0.8 | 0.83 ± 0.04 | 4.7 | 0.79 ± 0.02 | 2.5 |
| 2.5 | 2.39 ± 0.15 | 6.2 | 2.35 ± 0.05 | 2.0 |
| CTP | ||||
| 20 | 18.0 ± 0.6 | 3.1 | 18.6 ± 0.3 | 1.6 |
| 40 | 36.8 ± 3.0 | 8.1 | 36.6 ± 2.4 | 6.6 |
| 150 | 136 ± 2 | 1.5 | 135 ± 4 | 2.6 |
| UTP | ||||
| 40 | 36.8 ± 1.1 | 2.9 | 38.8 ± 0.9 | 2.5 |
| 80 | 76.2 ± 2.9 | 3.9 | 82.1 ± 8.0 | 9.8 |
| 300 | 289 ± 6 | 2.2 | 303 ± 15 | 5.0 |
| ATP | ||||
| 100 | 104 ± 5 | 4.7 | 106 ± 5 | 4.5 |
| 200 | 180 ± 7 | 3.7 | 173 ± 2 | 1.0 |
| 750 | 684 ± 34 | 4.9 | 654 ± 28 | 4.3 |
| GTP | ||||
| 20 | 17.0 ± 0.8 | 4.6 | 18.7 ± 2.4 | 12.7 |
| 40 | 37.6 ± 1.6 | 4.2 | 34.6 ± 1.1 | 3.1 |
| 150 | 154 ± 6 | 3.6 | 152 ± 1.2 | 0.8 |