Figure 6.
Genomic context of sRNA genes. (A) Genomic organization of the sR101 (blue), sR21 (red) and sR100 (yellow) genes in the six species of Pyrobaculum. The 16S rRNA phylogenetic tree with Tte as the outgroup, is illustrated on the left; the sRNA gene locations above a bp distance scale is illustrated to the right for the Pyrobaculum species. There is no representative of the sR100 gene in Pne, Pog, Par and in Par and Pog there is an ∼200 nt insertion between the sR101 and sR21 genes. (B) Linkage of tRNA and sRNA genes. In Pae, Pis and Pne the sR44 genes (green arrows) are located 8 nts or less from the 3′ end of a tRNAMet gene (black arrows). In Pog and Par the distance between the tRNAMet and sR44 gene is increased to about 100 nts and appear to be expressed from separate promoters. In Pca, sR44 is located ∼13 Kbps downstream of the tRNAMet gene. There is no representative of the sR44 family in Tte.