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. 2018 May 9;46(11):5737–5752. doi: 10.1093/nar/gky306

Figure 2.

Figure 2.

Expression of 5′-extended pre-miR-HSUR4 in HCT116 cells with Drosha-, Dicer- or XPO5-KO. (A) pre-miR-HSUR4 with various 5′ extensions. Extension sequences are indicated, with dashed lines representing no sequences. Sequence of the WT pre-miR-HSUR4 containing 2 nt extension at the 5′ end and 1 nt [or 2 nt, U(C)] 3′ overhang is illustrated on the right. (B) Northern blot analyses of miR-HSUR4-5p and miR-HSUR4-3p from HCT116 cells with protein knockout as indicated. Cells were transfected with plasmids encoding different 5′-extended pre-miRNAs as well as EBER1, which is an Epstein-Barr virus encoded Pol III transcript serving as a transfection control. In the upper panel, open triangles point to different pre-miR-HSUR4s, while the solid triangles point to miR-HSUR4-5p. The asterisk (*) marks the ∼22 nt miR-HSUR4-5p resulting from a secondary Dicer cleavage (see Supplementary Figure S9).