Selection of inhibitor by measuring Pho4-GFP localization with static microscopy. The strains tested [EY0821 (pho85Δ), EY0825 (PHO85WT), and EY0823 (PHO85F82G)] were analyzed 15 min after treatment with inhibitor. A representative field of cells is shown for each condition. 2-NM PP1, 4-amino-1-tert-butyl-3-(2′-naphthylmethyl)pyrazolo[3,4-d]pyrimidine; 4-me PP1, 4-amino-1-tert-butyl-3-(1′-naphthyl-4′-methyl)pyrazolo[3,4-d]pyrimidine.