Figure 2.

Analysis of Pho5 activity induced by inhibitor treatment using the liquid phosphatase assay. Units of activity were calculated by dividing the measured OD₄₂₀ by the OD₆₀₀ of the yeast suspension used in the assay and are displayed on the vertical axis. Time after inhibitor addition is shown on the horizontal axis. The strains and treatments shown are: EY0822 (pho85Δ) + DMSO (□); EY0824 (PHO85^F82G) + 10 μM 1-Na PP1 (■), + 5 μM 1-Na PP1 (▴), + 1 μM 1-Na PP1 (crosses), + 0.5 μM 1-Na PP1 (⧫), or + DMSO (◊); and EY0826 (PHO85^WT) + 10 μM 1-Na PP1 (▵). Each point shown is the mean of three independent experiments, and the error bars indicate two standard deviations.