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Perfusion chamber fluorescence microscopy of Pho4-GFP localization in response to treatment with 10 μM 1-Na PP1. A single representative field of cells is shown through the course of the experiment. (A) Pretreatment. (B) After 10 min in 10 μM 1-Na PP1. (C) Five minutes after removal of 1-Na PP1. (Magnification: ×2,000.)
