Figure 2.

A. Immunoblot analyses of rhPAPPA2 expression and proteolytic activities. (A) Conditioned media (CM) from vector-transfected (lane 1) or pCMV6-PAPPA2–transfected (lane 2) HEK293 cells. Lane 3, rhPAPPA2 after concentration. (B) Secreted intact IGFBP-3 in media conditioned by human dermal fibroblasts was incubated with or without rhPAPPA2 (~20 ng) for 24 h at 37°C. Addition of rhPAPPA2 resulted in cleavage of IGFBP-3. (C) rhPAPPA2 was incubated for 48 h at 37°C with rhIGFBP5. Immunoblot showing cleavage of IGFBP-5 in the presence of rhPAPPA2.