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. 2018 Apr 30;46(11):5547–5560. doi: 10.1093/nar/gky281

Figure 7.

Figure 7.

The expression of KDM4A, KDM4C and WDR5 is dys-regulated in SLE B cells. (A, B) mRNA levels of KDM4A (A) and KDM4C (B) in [anti-IgM + IL-21 + anti-CD40 + BAFF]-stimulated CD19+ human B cells isolated from peripheral blood of healthy donors and SLE patients. (C) Immunoblotting showing the levels of H3K9me2/me3 histone marks at the indicated time point of [anti-IgM + IL-21 + anti-CD40 + BAFF]-stimulated CD19+ human B cells isolated from three healthy donors and three SLE patients. (D) mRNA levels of WDR5 in normal and SLE B cells as described in (A and B) at indicated days. (E) mRNA levels of various CDKNs in normal and SLE B cells as described in (A and B) at day 3. (F) WDR5 mRNA levels in healthy donor peripheral blood B cells stimulated with anti-IgM + IL-21 + anti-CD40 + BAFF and treated with 8-HQ (200 μM) or DMSO for 3 days. (G) Analysis showing PKH26 dilution in stimulated healthy donor peripheral blood B cells treated with either 8-HQ or DMSO for 3 days. Results in (A), (B), (D), (E) and (F) represent the mean ± SEM (n = 10). *P < 0.05, **P < 0.01, ***P < 0.005 (Student's t-test).