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. 2018 Mar 13;46(11):e64. doi: 10.1093/nar/gky184

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© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Distribution of localized Atto647N labels on the plasmids for the standard two-step mTAG labeling reaction (light-gray) compared with the one-pot labeling reaction (black). The average number of fluorophores per plasmid in the standard reaction is 1.3 as compared to 3.5 for the one-pot reaction.