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(A) Image showing the fluorogenic Suzuki coupling of IU-labeled transcript 4 with boronic esters 16 and 17. The samples were irradiated using 365 nm light source. (B) Emission spectra (1 μM) of substrates (very weakly emissive) and RNA ON products 16a′ and 17a′ (highly emissive). (C) Synthesis of RNA ONs 17a′, 19a′ and 20a′, containing emissive nucleoside 18 in-between different flanking bases, from respective iodo-labeled RNA ON transcripts by post-transcriptional Suzuki coupling.
