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. 2018 Mar 29;46(11):5441–5454. doi: 10.1093/nar/gky234

Figure 5.

Figure 5.

LSD1 inhibition facilitates Dex-induced expression of oxidative genes. (A) Effects of Dex and S2101 on expression of oxidative metabolism genes (n = 3). C2C12 cells were cultured in differentiation medium for 7 days in the presence of Dex (10−6 M) alone or with S2101 (10−5 M). Values shown are fold differences against vehicle-treated controls. (B) Expression of the Myh7 gene in Dex- and S2101-treated cells. Values shown are fold differences against vehicle-treated controls. (C and D) Expression of oxidative metabolism and Myh7 genes in C2C12 myotubes treated with Dex and S2101 during the early phase (C) or late phase (D) of differentiation (n = 4). RNA was extracted from C2C12 cells on day 7 after differentiation with the indicated treatments. (E) Enrichment of H3K4me1 at the Myh7 gene locus. ChIP analyses were performed using differentiating C2C12 cells (48 h) treated with Dex and S2101 (n = 3). Values are each shown as % input. (F) Enrichment of H3K4me2 at the promoter regions of oxidative metabolism genes. *P < 0.05, **P < 0.01. NS: no significant difference.