Figure 10.

Distribution of tomato-positive cells in H felis–infected Lgr5-Cre;H⁺/K⁺-Nog;Rosa26-Tom mice. One- to 2-month-old Lgr5-Cre;Rosa26-Tom mice and Lgr5-Cre;H⁺/K⁺-Nog;Rosa26-tdTom mice were treated with 1 intraperitoneal injection of tamoxifen (0.1 mg/g body weight) and inoculated with H felis 2 months after tamoxifen. Animals were analyzed 3 months after inoculation and 5 months after tamoxifen injection. Gastric paraffin sections of the lesser curvature of Lgr5-Cre;Rosa26-Tom and Lgr5-Cre;H⁺/K⁺-Nog;Rosa26-tdTom in the presence and absence of H felis (H. f.) were stained with anti-tomato (red fluorescent protein) primary antibodies and Alexa 555–conjugated secondary antibodies. Matching sections of the gastric mucosa of both noninfected and H felis–infected Lgr5-Cre;H⁺/K⁺-Nog;Rosa26-tdTom mice were stained with H&E. Similar results were observed in at least 3 other mice in each group. DAPI, 4′,6-diamidino-2-phenylindole.