Figure 4.

Expression of IF in cells with Lgr5 transcriptional activity. One- to 2-month-old Lgr5-Cre mice and Lgr5-Cre;Bmpr1a^flox-flox mice were treated with 1 intraperitoneal injection of tamoxifen (0.1 mg/g body weight) and inoculated with H felis 2 months after tamoxifen. Animals were analyzed 3 months after inoculation and 5 months after tamoxifen injection. Paraffin sections of the lesser curvature of Lgr5-Cre and of Lgr5-Cre;Bmpr1a^flox-flox mice in the presence and absence of H felis (H. f.) were stained with anti-IF primary antibodies and an Alexa 555–conjugated secondary antibody (red) together with Alexa 488–conjugated anti-GFP antibodies (green). Scale bar: 25 μm. Similar results were observed in at least 1 other mouse and in 6 other mice in the H felis–infected Lgr5-Cre;Bmpr1a^flox-flox group. DAPI, 4'6-diamidino-2-phenylindole.