Figure 6.

Decreased parietal cells and increased cell proliferation in Helicobacter-infected Lgr5-Cre;Bmpr1a^flox-floxmice. One- to 2-month-old Lgr5-Cre mice and Lgr5-Cre;Bmpr1a^flox-flox mice were treated with 1 intraperitoneal injection of tamoxifen (0.1 mg/g body weight) and inoculated with H felis 2 months after tamoxifen. Animals were analyzed 3 months after inoculation and 5 months after tamoxifen injection. Paraffin sections of the lesser curvature of Lgr5-Cre mice and of Lgr5-Cre;Bmpr1a^flox-flox mice, in the presence and absence of H felis (H. f.), were stained with an (A) anti-H⁺,K⁺-ATPase α-subunit primary antibody and an Alexa 488–conjugated secondary antibody (green), and with an (C) anti-Ki67 primary antibody and an Alexa 555–conjugated secondary antibody (red). Scale bars: 100 μm. Bars represent the number of (B) H⁺,K⁺-ATPase α-subunit and of (D) Ki67-positive cells detected in Lgr5-Cre mice and in Lgr5-Cre;Bmpr1a^flox-flox mice in the presence and absence of H felis. Values are shown as means ± SE. Numbers in parenthesis indicate the number of animals used in each group. *P < .05 vs Lgr5-Cre mice. **P < .05 vs Lgr5-Cre;Bmpr1a^flox-flox mice. DAPI, 4'6-diamidino-2-phenylindole.