Figure 7.

Development of SPEM in H felis–infected Lgr5-Cre;Bmpr1a^flox-floxmice. One- to 2-month-old Lgr5-Cre mice and Lgr5-Cre;Bmpr1a^flox-flox mice were treated with 1 intraperitoneal injection of tamoxifen (0.1 mg/g body weight) and inoculated with H felis 2 months after tamoxifen. Animals were analyzed 3 months after inoculation and 5 months after tamoxifen injection. Gastric paraffin sections of the lesser curvature of Lgr5-Cre and of Lgr5-Cre;Bmpr1a^flox-flox mice in the presence and absence of H felis (H. f.) were stained with anti-IF primary antibodies and Alexa 555–conjugated secondary antibodies (red) together with Alexa 488–conjugated GS II (green). Gastric paraffin sections of the lesser curvature of H felis–infected Lgr5-Cre;Bmpr1a^flox-flox mice were stained with anti-TFF2 primary antibodies and an Alexa 488–conjugated secondary antibody. Scale bar: 100 μm. Similar results were observed in at least 3 other mice in each group. DAPI, 4′,6-diamidino-2-phenylindole.