Figure 8.

Expression of CD44 in H felis–infected Lgr5-Cre;Bmpr1a^flox-floxmice. One- to 2-month-old Lgr5-Cre mice and Lgr5-Cre;Bmpr1a^flox-flox mice were treated with 1 intraperitoneal injection of tamoxifen (0.1 mg/g body weight) and inoculated with H felis 2 months after tamoxifen. Animals were analyzed 3 months after inoculation and 5 months after tamoxifen injection. (A) Paraffin sections of the lesser curvature of Lgr5-Cre and Lgr5-Cre;Bmpr1a^flox-flox mice in the presence and absence of H felis (H. f.) stained with anti-CD44 primary antibodies and a biotin-conjugated secondary antibody. The magnified window shows intense CD44 staining of epithelial cells in H felis–infected Lgr5-Cre;Bmpr1a^flox-flox mice. Scale bar: 100 μm, 50 μm in the magnified window. Arrows point to CD44^+ve cells. (B) Paraffin sections of the lesser curvature of noninfected and H felis–infected Lgr5-Cre;Bmpr1a^flox-flox mice stained with anti-CD44v9 primary antibodies and Alexa 488–conjugated secondary antibodies. The magnified window shows CD44v9 staining of epithelial cells at the base of glands in H felis–infected Lgr5-Cre;Bmpr1a^flox-flox mice. Scale bar: 100 μm, 50 μm in the magnified window. Arrows point at CD44v9^+ve cells. (C) Paraffin sections of the lesser curvature of H felis–infected Lgr5-Cre;Bmpr1a^flox-flox mice stained with anti-CD44 primary antibodies and Alexa 594-conjugated secondary antibodies (red) together with Alexa 488–conjugated GSII (green), anti-CD44 primary antibodies and Alexa 488–conjugated secondary antibodies (green) together with IF antibodies and Alexa 555–conjugated secondary antibodies (red) and anti-CD44 primary antibodies and Alexa 488–conjugated secondary antibodies (green) together with anti-Ki67 antibodies and Alexa 555–conjugated secondary antibodies (red). Similar results were observed in at least 2 other mice in each group. Scale bar: 50 μm. DAPI, 4′,6-diamidino-2-phenylindole.