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. 2018 Jan 12;5(4):499–522. doi: 10.1016/j.jcmgh.2018.01.006

Figure 9.

Figure 9

Bile acid cytotoxicity and protective role of intestinal Nrf2 (Cap ‘n’ collar isoform-c [cncC]) in Drosophila. (A) Relative survival of 5-day-old adult Drosophila in response to feeding CDCA or deoxycholic acid (DCA). Log-rank test for CDCA or DCA vs sucrose, P < .001, n = 30–44 per group. (B) Relative survival of 5-day-old adult Drosophila in which the levels of cncC (UAS-CncCIR) are diminished; log-rank test for myolA-GAL4 vs myolA-GAL4, UAS-cncCIR, P < .0001, n = 50 per group. (C) Detection of ARE-dependent GFP expression in the midgut of gstD-gfp adult Drosophila fed sucrose, sucrose plus chenodeoxycholic acid, or sucrose plus paraquat. (D) ROS generation in midgut after ingestion of sucrose or sucrose plus 25 mmol/L CDCA. ROS were detected by oxidation of the hydrocyanine ROS-sensitive dye, Hydro-Cy3. Images within a panel were taken at the same confocal settings: original magnification, 20×.