Supplementary Figure 1.

Mutant KRAS exosomes enhance cell-cycle progression and proliferation of DKs-8 cells cultured in low-glucose medium. DKs-8 cells were incubated with DKs-8 or DKO-1 exosomes or mock-treated (A) for 48 hours or (B) for times shown. Cell-cycle state was assayed by Hoechst staining followed by flow cytometric analysis (n = 3 in triplicate). (C) Growth of exosome-treated DKs-8 cells after 120 hours as determined by relative fluorescence intensity (RFU) of stained nuclei. (A and C) *P < .05 for comparison indicated. (B) *P < .05 for pairwise comparisons between DKO-1 exosomes vs DKs-8 exosomes or mock treatment.