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. 2018 Jan 31;5(4):627–629.e6. doi: 10.1016/j.jcmgh.2018.01.013

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© 2018 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Supplementary Figure 2 — Characterization of exosome effects in vitro. (A) Normal mouse colonoid cultures were characterized by antibody staining as indicated. Most epithelial cells express non–cell-surface E-cadherin (E-Cad; green); smooth muscle actin (SMA; red) marks pericryptal fibroblasts. T-antigen (green) indicates the presence of young adult mouse colon (YAMC) cells. Na/K adenosine triphosphatase (ATPase) (green) marks epithelial cells separate from Pan-Cadherin–expressing cells. Most epithelial cells express cytokeratin (CYT) 8/18 (green). (B) Representative images for panel C. Redox ratios for (C) normal mouse colonoids cultured in Matrigel, (D) normal mouse colonic cells cultured on plastic, and (E) DKs-8 cells exposed for 48 hours to the treatments indicated. Data are mean normalized redox ratio ± SEM. *P < .05.