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Production of proinflammatory cytokines by human PBMCs. Human PBMCs were incubated with 100 ng/mL of TcdA, TcdB, or TcdB-L543A for 24 hours. The culture supernatants were collected for cytokine assays using the V-PLEX Cytokine Panel 1 and Proinflammatory Panel 1 Kits.
