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. 2001 Oct 23;98(22):12596–12601. doi: 10.1073/pnas.221303298

Figure 1.

Figure 1

The GeneSwitch/UAS expression system in Drosophila. Driver lines expressing the transcriptional activator GeneSwitch in a tissue-specific fashion are crossed to UAS-reporter lines with genomic inserts of a target gene fused to five GAL4-binding sites arrayed in tandem (5× UAS). In the absence of an activator, the GeneSwitch protein is expressed in target tissues but remains transcriptionally silent (black); Gene X is therefore not expressed. However, after systemic application of RU486 (red), the GeneSwitch protein becomes transcriptionally active (blue), mediating expression of gene X (green) in only those tissues expressing GeneSwitch. [Reproduced with permission from ref. 1 (Copyright 1993, The Company of Bioligists Limited).]