Table 1.
Catabolic rate of serum CN1 in the presence of inhibitors (n = 5).
Vmax (µmol/mg/h) | Km (mM) | Efficiency (Vmax/Km) | |
---|---|---|---|
Control | 4.2 ± 0.4 | 0.8 ± 0.1 | 5.2 ± 0.2 |
Cysteine (1 mM) | 1.8 ± 0.2* | 1.0 ± 0.2 | 1.6 ± 0.2* |
N-acetylcysteine (1 mM) | 2.4 ± 0.2* | 1.1 ± 0.3 | 2.0 ± 0.3* |
GSH (1 mM) | 2.9 ± 0.4* | 0.9 ± 0.2 | 3.2 ± 0.4* |
GSSG (1 mM) | 4.1 ± 0.5 | 0.9 ± 0.3 | 4.6 ± 0.4 |
l-Glutamic acid (1 mM) | 4.2 ± 0.5 | 0.8 ± 0.2 | 5.2 ± 0.3 |
Glycine (1 mM) | 4.1 ± 0.4 | 0.7 ± 0.3 | 5.8 ± 0.4 |
Efficiency of recombinant CN1 activity were calculated by the ratio of Vmax and Km.
p < .05 compared to control.