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Detection of apoptosis induced by compound 12b in non-small cell lung cancer cell line A549 using Annexin V-FITC assay. Cells were visualised by fluorescence microscope at 40× magnification before and after treatment with the concentration of 2 × IC50 for 48 h. PI staining was used as a nuclear marker. Shown here are bright-field images (left micrographs) and late apoptotic/primary necrotic cells (right micrographs).
