Table 2.

Annexin V assay for apoptosis detection.

A549	Control (%)	12b (%)
Secondary necrotic cells	0	2.39
Early apoptotic cells	0.52	31.14
Viable cells	99.48	46.71
Late apoptotic/Primary necrotic cells	0	19.76

^aThe percentages of viable cells (PI−/Ann V−), early apoptotic cells (PI−/Ann V+), late apoptotic/primary necrotic cells (PI+/Ann V+) and secondary necrotic cells (PI+) after 48 h treatment with compound 12b at 2 × IC₅₀ value are shown.