Figure 2.

The binding affinity of Rimonabant and its analogs on CBR (A) and the MOR (B), DOR (C), KOR (D) and CBR binding affinity of 5, 9 and 10 (Tyr-D-Ala-Gly-Phe-NH₂) (E) in competition binding experiments. Figures represent the specific binding of [³H]WIN55 212–2, [³H]DAMGO, [³H]IleDelt II and [³H]HS665 in percentage in the presence of increasing concentrations (10 ⁻¹¹–10⁻⁵ M) of the indicated unlabeled ligands performed in rat (A, B and E) or in guinea pig (D) whole brain membrane homogenates. “Total” on the x-axis indicates the total specific binding of radioligand, which is measured in the absence of the unlabeled compounds. The level of total specific binding was defined as 100% and is presented with a dotted line. Points represent means ± SEM for at least three experiments performed in duplicates.