Table 2.

Activation of hCA I, hCA II, VchCAβ, Rv3273, VchCAγ and BpsCAγ with tripeptides 1–6 and simple amino acid derivatives, by a stopped-flow, CO₂ hydrase assay, at 25 °C and pH 8.4³³.

KA (µM)*
Activator	hCA Ia	hCA IIa	VchCAβ	Rv3273	VchCAγ	BpsCAγ
1	>50	>50	3.52 ± 0.18	8.45 ± 0.11	14.7 ± 0.21	10.1 ± 0.09
2	>50	>50	1.16 ± 0.05	6.29 ± 0.14	5.84 ± 0.15	1.63 ± 0.12
3	>50	>50	1.15 ± 0.10	4.32 ± 0.08	11.9 ± 0.42	3.75 ± 0.15
4	>50	>50	0.21 ± 0.07	15.8 ± 0.76	12.9 ± 0.61	6.18 ± 0.30
5	>50	>50	7.16 ± 0.34	18.1 ± 1.02	10.6 ± 0.74	0.95 ± 0.09
6	>50	>50	4.18 ± 0.23	9.40 ± 0.62	2.74 ± 0.16	5.24 ± 0.30
l-Asp	5.20	>50	9.87 ± 0.41	10.1 ± 0.84	8.95 ± 0.46	10.7 ± 0.85
l-Asn	11.3	>50	>50	10.0 ± 0.71	6.37 ± 0.29	0.98 ± 0.08
l-Glu	6.43	>50	0.69 ± 0.05	>50	6.48 ± 0.50	3.25 ± 0.17
l-Gln	>50	>50	18.1 ± 0.94	21.6 ± 1.1	9.21 ± 0.82	6.15 ± 0.30
l-Hisa	0.03	10.9	20.3	18.2	1.01	24.7
l-Phea	0.07	0.013	15.4	30.6	0.73	1.73

*Mean ± standard error, from three different assays.

^aFrom Refs.^28–31.