Table 1.
Inhibition constants of neohesperidin dihydrochalcone, rutin and phloretin for the reduction of L-idose, or HNE determined at different DMSO concentrations.
| Ki (µM) |
Ki′ (µM) |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Substrate | Inhibitor | Inhibition model | No DMSO | DMSO 40 mM | DMSO 100 mM | DMSO 200 mM | No DMSO | DMSO 40 mM | DMSO 100 mM | DMSO 200 mM |
| L-idose | NHDC | Mixed | 93 ± 17 | 114 ± 15 | 125 ± 16 | 194 ± 17 | 292 ± 17 | 286 ± 53 | 230 ± 35 | 283 ± 2 |
| HNE | NHDC | Uncompetitive | – | – | – | – | 122 ± 19 | 134 ± 11 | 150 ± 22 | 199 ± 6 |
| L-idose | Rutin | Mixed | 17.8 ± 3.0 | 20.3 ± 1.1 | 24.8 ± 3.8 | 45.6 ± 5.5 | 9.3 ± 1.1 | 7.6 ± 1.2 | 11.7 ± 0.5 | 12.7 ± 2.0 |
| HNE | Rutin | Uncompetitive | – | – | – | – | 9.2 ± 0.9 | 10.5 ± 2.0 | 12.8 ± 1.9 | 16.5 ± 1.9 |
| – | – | – | – | |||||||
| L-idose | Phloretin | Mixed | 66.6 + 5.8 | 85.4 + 9.0 | 98.9 + 11.7 | 120.8 + 43.1 | 52.5 + 3.8 | 62.9 + 2.3 | 50.3 + 4.8 | 60.9 + 2.1 |
| HNE | Phloretin | Mixed | 60.5 + 7.2 | 79.0 + 23.5 | 94.8 + 12.1 | 106.8 + 12.3 | 56.5 + 4.5 | 64.7 + 1.4 | 73.4 + 7.5 | 99.5 + 7.3 |