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. 2017 Oct 2;32(1):1265–1273. doi: 10.1080/14756366.2017.1378190

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© 2017 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Exposure of HEp-2 cells to Neem oil-based NEs. HEp-2 cells were treated with NEs or free Neem oil as described (see Methods). The OD values obtained by MTT assay for treated cells were converted into numbers of cells on a standard curve and expressed as percentages of untreated controls. Bars represent the mean of three independent experiments ± SD. *p < .05 (Panel A). Fluorescence microscopy images of HEp-2 control cells exposed to free Nile red (Panel B) and HEp-2 cells exposed to Nile red-loaded NEs for 7 (Panel C) and 24 h (Panel D) obtained by fluorescence microscopy as described (see Methods). Scale bar: 10 μm.