Table 2.
In vitro COX-1/COX-2 enzyme inhibition assay.
| Compd No. | IC50 (μM)a |
SIb | |
|---|---|---|---|
| COX-1 | COX-2 | ||
| Celecoxib | >50 | 0.16 ± 0.011 | >312.5 |
| 2b | >50 | 0.66 ± 0.052 | >75.8 |
| 3b | >50 | 0.69 ± 0.057 | >72.5 |
| 6a | >50 | 0.25 ± 0.017 | >200.0 |
| 7a | >50 | 0.22 ± 0.019 | >227.3 |
| 7b | >50 | 0.49 ± 0.044 | >102.0 |
| 8a | >50 | 0.20 ± 0.016 | >250.0 |
| 8b | >50 | 0.60 ± 0.055 | >83.3 |
aIC50 value is the compound concentration required to produce a 50% inhibition of COX-1 or COX-2, calculated as the mean of two determinations using the ovine COX-1/COX-2 assay kit (catalog no. 560101, Cayman Chemicals Inc., USA); the deviation from the mean is <10% of the mean value.
bSelectivity index (COX-1 IC50/COX-2 IC50).