Fig. 4.

After ETS1 knocking down in 231/DDP cell line. a MTT assay is used to evaluate the chemo-sensitivity of 231/wt cell line and 231/DDP cell line transfected by shETS1 to different drugs of DDP/CTX/Paclitaxel/DOX after 48 h treatment (N = 3, **P < 0.001, *P < 0.01); b difference on intracellular accumulation of DDP in 231/wt and 231/DDP cell lines after siETS1 transfection. ICP-MS method was used to detect intracellular DDP accumulation in cells treated with DDP 1 μg/mL for 24 h, **P < 0.001; c, d annexin V/PI assay is used to detect apoptosis in 231/DDP-NC and 231/DDP-KD cell lines with different concentration of DDP; e proteins expression after ETS1 knocking down