Figure 3.

Compound 2 demonstrates antiproliferative effects in SCCHN cells (A) IC₅₀ of BHY (EGFR low) and FADU (EGFR int) cell lines incubated with the indicated concentration of compound 2. Cells were incubated with compound 2 for 48 h and then subjected to PI staining and FACS analysis. PI-positive cell fraction as measure of cell death is reported. (B) Four SCCHN cell lines were incubated with 100 μM of compound 2 and counted on the indicated days. Experiments in triplicates, fold growth shown. (C) EGFR expression of SCCHN cell lines. EGFR high and low as detected by western blot analysis (D) The SCCHN cell lines were incubated with 100 μM of compound 2 for 72 h. Percent PI positive cells are given. Experiments in triplicates.