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. 2018 Jun 20;11:170. doi: 10.1186/s13068-018-1167-z

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — The percentage of the G1 transcripts satisfying each of the six thresholds defined along the x-axis. The four thresholds marked as a, b, c, d, are for high-quality alignment in human, maize, Amborella, and PacBio tutorial Iso-Seq analysis, respectively [18, 26, 27]