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Published in final edited form as: Cancer Prev Res (Phila). 2014 Sep 22;7(11):1149–1159. doi: 10.1158/1940-6207.CAPR-14-0091

The effects of honokiol on mitochondria function in H226 and H520 cells. A, Mitochondrial function upon honokiol treatment. Cells (20,000 cells per well) seeded in V7 culture plates were treated with honokiol at indicated concentrations for 4 hours, the cells were then washed with unbuffered media as described. Five baseline OCR and ECAR measurements were then taken before injection of oligomycin (1 μg/mL), to inhibit ATP synthase, FCCP (1–3 μmol/L) to uncouple the mitochondria and yield maximal OCR, and antimycin A (10 μmol/L) to inhibit complex III and mitochondrial oxygen consumption. The effects of honokiol on basal OCR, ATP-linked OCR and ECAR are shown. B, Honokiol caused ATP depletion in NSCLC cells. Cells were treated with honokiol for different time. Intracellular ATP levels were monitored using a luciferase-based assay. Data shown are the means ± SE, n=4 per treatment group. *, P < 0.05 versus control. C, Honokiol activated AMPK pathway. Left panel, western blotting analysis of AMPK pathway. Right Panel, optical band density analysis.

The effects of honokiol on mitochondria function in H226 and H520 cells. A, Mitochondrial function upon honokiol treatment. Cells (20,000 cells per well) seeded in V7 culture plates were treated with honokiol at indicated concentrations for 4 hours, the cells were then washed with unbuffered media as described. Five baseline OCR and ECAR measurements were then taken before injection of oligomycin (1 μg/mL), to inhibit ATP synthase, FCCP (1–3 μmol/L) to uncouple the mitochondria and yield maximal OCR, and antimycin A (10 μmol/L) to inhibit complex III and mitochondrial oxygen consumption. The effects of honokiol on basal OCR, ATP-linked OCR and ECAR are shown. B, Honokiol caused ATP depletion in NSCLC cells. Cells were treated with honokiol for different time. Intracellular ATP levels were monitored using a luciferase-based assay. Data shown are the means ± SE, n=4 per treatment group. *, P < 0.05 versus control. C, Honokiol activated AMPK pathway. Left panel, western blotting analysis of AMPK pathway. Right Panel, optical band density analysis.