Table 1.
Effect of AntiOxBEN3 on mitochondrial bioenergetics: mitochondrial respiratory control ratio (RCR); ADP phosphorylation efficiency (ADP/O); and approximate transmembrane electric potential (ΔΨ).
| AntiOxBEN3 |
|||||
|---|---|---|---|---|---|
| Mitochondrial Bioenergetics | Control | 2.5 µM | 5 µM | 10 µM | |
| Glut/Mal | Maximum potential (app. ΔΨ in − mV) | 229.8 ± 17.4 | 221.1 ± 20.2 | 221.4 ± 22.6 | 227.5 ± 26.3 |
| RCR | 7.3 ± 0.6 | 3.9 ± 0.5** | 3.9 ± 0.6** | 3.07 ± 0.6**** | |
| ADP/O | 2.6 ± 0.1 | 2.3 ± 0.2 | 2.3 ± 0.1 | 2.0 ± 0.2* | |
| Succinate | Maximum potential (app. ΔΨ in − mV) | 186.1 ± 6.6 | 203.6 ± 16.6 | 205.3 ± 19.4 | 207.9 ± 19.3 |
| RCR | 4.1 ± 0.3 | 4.1 ± 0.5 | 4.3 ± 0.7 | 3.9 ± 0.4 | |
| ADP/O | 1.5 ± 0.1 | 1.6 ± 0.1 | 1.6 ± 0.1 | 1.7 ± 0.1 | |
Effect of AntiOxBEN3 on approximate ΔΨ, RCR and ADP/O of energised RLM (5 mM glutamate/2.5 malate or 5 mM succinate). Values are means ± SEM of five independent experiments. Statistically significant compared with control using Student’s two tailed t-test. Significance was accepted with *p < .05, **p < .01, ****p < .0001.