Table 2.

Effect of different concentrations of compound 1 on V_max, K_m, and the K_ik to K_iv ratio using PTP1B and α-glucosidase.

Enzyme	[I] µM	Vmax	Km	Kik/Kiv
PTP1Ba	0	0.016	0.712	//
	0.78	0.012	1.193	2.660
	1.56	0.009	1.779	3.335
	3.13	0.007	2.248	3.694
α-Glucosidaseb	0	0.157	157.68	//
	12.5	0.117	154.47	12.42
	25	0.082	153.76	19.34
	50	0.054	153.91	27.48

V_max and K_m values were calculated according to Lineweaver–Burk from the data shown in Figures 3, 4 and supplemental. K_ik/K_iv ratio was calculated according to Yang et al.²¹

^ap-Nitrophenyl phosphate (pNPP) as substrate.

^bp-Nitrophenyl glucopyranoside (pNPG) as substrate.