Figure 2.

Alignment of α-CA sequences from Mytilus galloprovincialis (MgaCA, Accession number: ALF62133.1) Corallium rubrum (CruCA4, Accession number: KU557746), Homo sapiens I (hCAI, Accession number: NP001729) and II (hCAII, Accession number: P00918), hCA I numbering system was used. The zinc ligands (His94, 96 and 119) and the gate-keeper residues (Glu106 and Thr199) are conserved in aligned sequences; while the proton shuttle residue (His64) is preserved only in the human enzymes. The asterisk (*) indicates identity at all aligned positions; the symbol (:) relates to conserved substitutions, while (.) means that semi-conserved substitutions are observed. Multialignment was performed with the program Muscle, version 3.1.