Table 1.

Kinetic parameters for the CO₂ hydration reaction catalysed by the rec-MgaCA, the purified native mussel CA (MgaCA), the Homo sapiens CA isoforms (hCA I and hCA II) and coral CA isoforms (SpiCA1 and SpiCA2 from Stylophora pistillata; CruCA4 from Corallium rubrum). Acetazolamide (AAZ) inhibition data are also shown.

Enzyme	Class	kcat (s−1)	kcat/KM (M−1 × s−1)	KI (acetazolamide) (nM)
hCA I	α	2.0 × 105	5.0 × 107	250
hCA II	α	1.4 × 106	1.5 × 108	12
SpiCA1	α	3.1 × 105	4.6 × 107	16
SpiCA2	α	5.6 × 105	8.3 × 107	74
CruCA4	α	2.4 × 105	5.2 × 107	450
MgaCA	α	4.1 × 105	3.6 × 107	380
rec-MgaCA	α	4.2 × 105	3.5 × 107	361

Errors in the range of ±5% of the reported data from three different assays.