Table 1.
Summary of the alkaline protease from A. pallidus C10 purification procedure.
| Purification methods | Purification steps | Total activity(U) | Total protein(mg) | Specific activity(U/mg) | Yield (%) | Purificationfold |
|---|---|---|---|---|---|---|
| DE52 anion exchange chromatography | Culture supernatant | 2148 | 42960 | 0.056 | 100 | 1 |
| (NH4)2SO4 precipitation and dialysis | 283.6 | 1610 | 0.176 | 13.02 | 3.14 | |
| Anion exchange chromatography | 76.3 | 280 | 0.272 | 26.9 | 4.85 | |
| Probond affinity chromatography | Culture supernatant | 2473.8 | 40380 | 0.061 | 100 | 1 |
| (NH4)2SO4 precipitation and dialysis | 332.5 | 4088 | 0.081 | 13.44 | 1.33 | |
| Affinity chromatography | 65.04 | 61.05 | 1.057 | 19.56 | 17.32 |