Table 4.

The effect of organic solvents on A. pallidus C10 alkaline protease activity.

Organic solvents	Concentration (%)	Residual activity (%) (1h)	p Values	Residual activity (%) (24h)	p Values
Control	0	100	–	100	–
Methanol	15	68.42 ± 1.83	<0.0001 d	83.38 ± 1.77	0.0001 c
	25	114 ± 2.79	0.0009 c	50.27 ± 1.39	<0.0001 d
	50	80.24 ± 1.74	<0.0001 d	0	–
Ethanol	15	93.59 ± 1.12	0.0011 b	90.28 ± 1.98	0.0014 b
	25	107.8 ± 1.52	0.0009 c	75.47 ± 2.95	0.0002 c
	50	103.7 ± 2.07	0.0303 a	0	–
Acetone	15	86.73 ± 1.29	<0.0001 d	102.9 ± 1.92	0.0472 a
	25	88.50 ± 2.02	0.0008 c	72.67 ± 1.45	<0.0001 d
	50	72.74 ± 1.89	<0.0001 d	0	–
DMSO	15	84.73 ± 0.93	<0.0001 d	85.86 ± 3.65	0.0030 b
	25	78.10 ± 1.75	<0.0001 d	102.2 ± 1.53	0.0522ns
	50	95.33 ± 2.19	0.0299 a	0	–
Butanol	15	93.74 ± 2.09	0.0091 b	58.88 ± 2.20	<0.0001 d
	25	73.66 ± 1.85	<0.0001 d	106.5 ± 3.21	0.0217 a
	50	52.01 ± 2.44	<0.0001 d	0	–
Chloroform	15	95.94 ± 2.58	0.0724ns	74.56 ± 0.13	<0.0001 d
	25	85.02 ± 1.73	0.0002 c	96.41 ± 0.87	0.0056 b
	50	59.59 ± 1.44	<0.0001 d	0	–
Isopropanol	15	90.21 ± 1.91	0.0012 b	90.13 ± 1.73	0.0008 c
	25	84.46 ± 1.00	<0.0001 d	62.91 ± 2.42	<0.0001 d
	50	50.50 ± 1.038	<0.0001 d	0	–

p > 0.05 (not significant, ns).

^a p < 0.05.

^b p < 0.01.

^c p < 0.001.

^d p < 0.0001.