Table 3.
hCA I, II and mtCA 3 inhibition data of compounds 5 and 6 reported in the article, by a stopped-flow CO2 hydrase assay20.
| Compound | Ki (nM)a |
||
|---|---|---|---|
| hCA I | hCA II | mtCA 3 | |
| 5a | 306 | 282 | 1800 |
| 5b | 441 | 500 | 486 |
| 5c | 634 | 2850 | 732 |
| 5d | 93.4 | 426 | 175 |
| 5e | 175 | 42.3 | 138 |
| 5f | 445 | 433 | 215 |
| 5g | 174 | 578 | 147 |
| 5h | 280 | 424 | 186 |
| 5i | 276 | 373 | 233 |
| 5j | 91.8 | 2020 | 157 |
| 5k | 440 | 392 | 127 |
| 5l | 760 | 810 | 1583 |
| 5m | 2374 | 5545 | >10,000 |
| 5n | 835 | 944 | 1288 |
| 5o | 898 | 3461 | 275 |
| 5p | 665 | 903 | 250 |
| 5q | 742 | 648 | 623 |
| 5r | 721 | 83.1 | 1592 |
| 5s | 738 | 73.6 | 265 |
| 5t | 842 | 5154 | 1735 |
| 5u | 798 | 126 | 1452 |
| 6a | 220 | 549 | >10,000 |
| 6b | 266 | 914 | >10,000 |
| 6c | 54.6 | 32.1 | 2145 |
| 6d | 316 | 418 | >10,000 |
| 6e | 1802 | 758 | 736 |
| 6f | 648 | 703 | 2128 |
| 6g | 327 | 255 | 254 |
| 6h | 292 | 483 | 2003 |
| 6i | 890 | 446 | 216 |
| 6j | 785 | 240 | 232 |
| AAZ | 250 | 12.1 | 104 |
AAZ was used as standard drug.
aMean from three different assays. The errors were in the range of ±10% of the reported values.