Table 3.
Viability assay of L. infantum promastigotes, cytotoxicity test using RAW 267.4 macrophage cells, and determining the selectivity index of NEs.
|
L. infantum |
|||||||
|---|---|---|---|---|---|---|---|
| Nanoemulsions | 3F | 3G | 3W | 5B | 5C | 5D | AMP6 |
| MIC1 | >128 | >128 | >128 | 64 | >128 | >128 | 4 |
| IC502 µM | 12.00 ± 4.3a | 10.72 ± 2.68a | 12.46 ± 1.35a | 3.47 ± 0.35c | 14.58 ± 0.88a | 51.70 ± 5.18b | 0.67 ± 0.10d |
| IC903 µM | n.d. | n.d. | n.d. | 52.03 ± 8.01a | n.d. | n.d. | 1.01 ± 0.03b |
| CC504 µM | 8.13 ± 1.19a | 6.77 ± 1.07b | 3.21 ± 0.55c | 6.51 ± 1.11b | 8.04 ± 1.33a | 6.75 ± 0.98b | 1.07 ± 1.49d |
| IS505 | 0.66 ± 0.17a | 0.77 ± 0.21a | 0.34 ± 0.12a | 2.01 ± 0.11b | 0.87 ± 0.22a | 0.11 ± 0.09c | 1.59 ± 0.01d |
The means compared by the Student–Newman–Keuls test.
n.d.: not determined.
1MIC: minimum inhibitory concentration.
2IC50: concentration which reduced 50% of the proliferation of L. infantum promastigote stage.
3IC90: concentration which reduced 90% of the proliferation of L. infantum promastigote stages.
4CC50: Concentration in μg·ml−1 cytotoxic for 50% of RAW 264.7 cells.
5SI: selectivity index = CC50/IC50.
6AMP: Amphotericin B.
a,b,c,dIn the lines: averages with equal letters do not differ statistically (p>.05).