Fig. 6.

EC treatment reduces microglia/macrophage activation 7 days after permanent focal ischemia. (A) Micrographs show Iba1-positive microglia/macrophages after treatment with vehicle or 15 mg/kg EC. Immunoreactive cells in the contralateral cortex of vehicle-treated mice show a quiescent phenotype compared with activated cells in the ischemic cortex (insets). EC at 15 mg/kg reduced numbers of activated Iba1-positive cells in the ischemic cortex, and the phenotype resembled inactivated contralateral cells (inset). (B) Quantification of Iba1 immunoreactivity shows reductions in WT mice treated with 10 or 15 mg/kg EC relative to vehicle-treated controls, whereas there was no difference in mice treated with 5 mg/kg. n = 8; **P < 0.01; ns, not significant; Cont, contralateral; Ips, ipsilateral; scale bars = 100 μm.