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. 2018 Jun 20;13(6):e0198013. doi: 10.1371/journal.pone.0198013

Fig 4. Nephrin deletion in adult mature glomerulus results in focal segmental glomerulosclerosis and proteinuria.

Fig 4

(A) Mice with tamoxifen-induced nephrin deletion develop proteinuria at 6–8 weeks following tamoxifen. (B) Masson Trichrome Verhoeff staining of mouse kidney sections at different time points following tamoxifen-induction showing development of focal and segmental glomerular scarring at 16 weeks following tamoxifen. Scale bars, 20μm. (C) Transmission EM images show normal foot process structure at 4 weeks following tamoxifen (Images were acquired at 10,400X and cropped for each panel). There is widespread foot process spreading at 8 weeks following tamoxifen. (D) Assessment of junction frequency shows decrease in the number of podocyte intercellular junction frequency at 8 weeks following tamoxifen. All results are representative of 4 sets of experiments with 5 animals in each group. Data are Mean ± SEM.