Fig 7. Nephrin has a half-life of 2.59 weeks at the membrane.

(A) Mouse kidney sections stained for nephrin using immunohistochemistry were analyzed for nephrin staining intensity at various time points following tamoxifen-induction using MetaMorph image analysis software. (B) Nephrin intensity at time = 0 weeks was plotted as 100%. There is rapid decline in nephrin staining in the initial 2 weeks followed by a slow decline over the next 14–16 weeks. The initial decline represents loss of new-nephrin production and represents nephrin that is primarily within the podocyte cell body. Nephrin staining after 2 weeks is primarily at the membrane (see S4 Fig). (C) Half-life analysis of nephrin at the membrane after the 2 weeks time point. Non-linear regression analysis (dotted line) shows a nephrin half-life 2.59 weeks at the membrane (R² = 0.83). The data is representative of 70–100 glomeruli per kidney from 3–4 animals in each group. Data are Mean ± SD.