Fig 9. Nphs1fl/fl,iCre(ER)T2 mice continue to be proteinuric following NTS injury.
(A) Coomassie blue stained SDS-PAGE gel show persistence of proteinuria following NTS single dose administration in Nphs1fl/fl,iCre(ER)T2 mice that received tamoxifen. (B) Urine albumin/creatinine ratio from Nphs1fl/fl,iCre(ER)T2 mice that were fed tamoxifen and normal chow following NTS injury. Most nephrin deleted mice did not survive beyond 8–10 days following NTS. (C) SEM images from Nphs1fl/fl,iCre(ER)T2 mice kidneys with and without tamoxifen induction following NTS injury show failure of recovery of nephrin-deleted podocytes at day 8 compared to control. The data is representative of 4 sets of experiments with 3 animals in each group. Sheep IgG was used as control. Data are Mean ± SEM. *P = Not significant, ** P <0.01, when compared between WT and Nephrin knock out in the NTS group.