Skip to main content
. 2018 Jun 20;13(6):e0199128. doi: 10.1371/journal.pone.0199128

Fig 4. The ectopic expression of α-1,2 fucosyltransferase FUT1 inhibits invadopodia in WM793 cells.

Fig 4

A, comparing the expression levels of FUT1 and FUT2 mRNA in normal skin, benign nevi, primary melanoma and metastatic melanoma in five microarray datasets. B, the ectopic expression of Flag-FUT1, as determined using anti-FLAG Western blotting. C, representative invadopodia assay images showing the effect of ectopically expressed FUT1 on invadopodium formation and gelatin degradation in WM793 cells D and E, quantitation of the effect of ectopically expressed FUT1 on the invadopodium number per cell (D) and the proportion of invadopodia positive cells (E) WM793. F and G, representative images (F) and quantitation data showing the effects of FUT1 overexpression in WM793 cells on Matrigel invasion. *, *** and **** indicates p< 0.05, 0.001 and 0.0001, as determined by two-tailed, two sample t-test (A, D, G) or two-tailed Fisher’s exact test (E). Numbers in parentheses indicates the number of cells used in quantitation. Representative results from at least three independent replicates were presented.