Key resources table.
Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
---|---|---|---|---|
Strain, strain background (M. musculus) |
Rem2 | EUCOMM | IKMC 92501 | Rem2tm1a(EUCOMM)Hmgu |
Strain, strain background (M. musculus) |
Flp | The Jackson Laboratory | RRID:IMSR_JAX009086 | B6.129S4-Gt(ROSA)26 Sortm1(FLP1)Dym/RainJ |
Strain, strain background (M. musculus) |
Emx1Cre | The Jackson Laboratory | RRID:IMSR_JAX:005628 | B6.129S2-Emx1tm1(cre)Krj/J |
Strain, strain background (M. musculus) |
PvalbCre | The Jackson Laboratory | RRID:IMSR_JAX:017320 | B6.129P2-Pvalbtm1(cre)Arbr/J |
Strain, strain background (M. musculus) |
VipCre | The Jackson Laboratory | RRID: IMSR_JAX:010908 | STOCK Viptm1(cre)Zjh/J |
Strain, strain background (M. musculus) |
TdT Flex | The Jackson Laboratory | RRID:IMSR_JAX:007909 | B6.Cg-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J |
Transfected construct (Adeno-associated virus) |
AAV-GFP | Penn Vector Core, USA | AV-1-PV1696 | AAV1.hSyn.eGFP.WPRE.bGH |
Transfected construct (Adeno-associaed virus) |
AAV-GFP-Cre | Penn Vector Core, USA | AV-1-PV1848 | AAV1.hSyn.HI.e GFP-Cre.WPRE.SV40 |
Antibody | polyclonal Anti-Rem2 | Santa Cruz | sc160722; RRID: AB_2179340 |
use 1:500 |
Antibody | monoclonal Anti-Bactin | Abcam | ab8226; RRID: AB_306371 |
use 1:5000 |
Software, algorithm | ImageJ software (Fiji) | NIH | RRID:SCR_003070 | |
Software, algorithm | ClampFit 10.2 | pClamp, Molecular Devices | RRID:SCR_011323 | |
Software, algorithm | Matlab | Mathworks | RRID:SCR_001622 | |
Software, algorithm | Reconstruct | Synapse Web | RRID:SCR_002716 | |
Software, algorithm | Volocity 3D Image Analysis Software |
Perkin Elmer | RRID:SCR_002668 |