Table 1. qPCR primers.
Gene | NCBI accession No. | Primer sequence |
ICAM-1 | NM_012967.1 | F:5′-CGGGAGATGAATGGTACCTACAA-3′ |
R:5′-TGCACGTCCCTGGTGATACTC-3′ | ||
TNF-α | NM_012675.3 | F:5′-ACAAGGCTGCCCCGACTAC-3′ |
R:5′-CTCCTGGTATGAAATGGCAAATC-3′ | ||
VEGF | NM_031836.3 | F:5′-GGGCCTCTGAAACCATGAACT-3′ |
R:5′-TGGTGGAGGTACAGCAGTAAAGC-3′ | ||
FGF2 | M22427.1 | F:5′-AAGGATCCCAAGCGGCTCTA-3′ |
R:5′-CGGCCGTCTGGATGGA-3′ | ||
GAPDH | NM_017008.4 | F:5′-ATGTATCCGTTGTGGATCTGACAT-3′ |
R:5′-CTCGGCCGCCTGCTT-3′ |
ICAM-1: Intercellular cell adhesion molecule-1; TNF-α: Tumor necrosis factor-α; VEGF: Vascular endothelial cell growth factor; FGF2: Fibroblast growth factor 2; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.