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. 2018 Jun 20;8:9398. doi: 10.1038/s41598-018-27662-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The activation of ERK1/2, p38, and associated kinases in high and low-responding phBEC cultures. Cells were considered “high responders” if their IL-8 induction was above the group mean (4.2 fold change) and low responders were below this group mean. Protein was collected from twelve donors (n = 12), six high and six low responders. (A) Blots from two representative donors (one high, one low) show the phosphorylation of ERK1/2 and an upstream (MEK1/2) and a downstream (RSK) kinase, as well as p38 and its upstream kinase (MKK4). Both sets of blots were run on the same gel, immunoblotted, and imaged at the same time. (B) Densitometry analysis was used to calculate the fold change in activation (normalized to filtered air control) for each donor. Median activation and interquartile range are shown for each group. Differences between high and low responders were determined via Mann-Whitney test. *p < 0.05.